Processing math: 100%
Sequence (5’ to 3’) ϵ260nm (M−1cm−1) DOI
AGGGCGGTGTGGGAATAGGGAA 233100 10.1074/jbc.M117.781906

       

Structure diagram of 5I2V

Structure diagram of 5I2V

     

Circular dichroism spectra of the 5I2V oligonucleotide (10 µM), acquired at 25°C in 0.4-cm path-length cuvettes

Circular dichroism spectra of the 5I2V oligonucleotide (10 µM), acquired at 25°C in 0.4-cm path-length cuvettes

 

$^{1}$H-NMR spectrum of the 5I2V oligonucleotide, acquired at 25°C in 100 mM TMAA (pH 7.0) + 1 mM KCl

1H-NMR spectrum of the 5I2V oligonucleotide, acquired at 25°C in 100 mM TMAA (pH 7.0) + 1 mM KCl

 

Folded fraction of the 5I2V oligonucleotide as a function of temperature, determined by UV-melting ($\lambda$ = 295 nm)

Folded fraction of the 5I2V oligonucleotide as a function of temperature, determined by UV-melting (λ = 295 nm)

Native ESI-MS spectra of the 5I2V oligonucleotide (10 µM)

Native ESI-MS spectra of the 5I2V oligonucleotide (10 µM)

 

Native ESI-MS spectra of the 5I2V oligonucleotide (10 µM), focused on the 5$^{-}$ charge state

Native ESI-MS spectra of the 5I2V oligonucleotide (10 µM), focused on the 5− charge state